This training will showcase tools, methods and technologies to interpret and determine protein structures. The training is aimed at novice scientists in the structural biology field but will also address scientists who are already more familiar with protein structure determination and would like to broaden their methodological basis. We will start this training with a comparison of the different technologies that are commonly used for structure determination. The technologies - NMR, Cryo-EM, X-ray crystallography, etc. - will be compared towards preparative work, timelines and interpretation. In a second session, the practical steps towards structure determination will be discussed. We will highlight how the preparative work that is needed for structure determination depends on the technology that is used. We will comment on the expected timelines and the sample requirements (amounts, purity, …) for the protein (-complex). In a next session, we will comment on good use of structural information, on correct interpretation and visualization of protein structures. This will all be further demonstrated in a point-by-point tutorial on screen. We will end the day with a keynote lecture to illustrate what was taught during the day in a biological story.